“Capacity building in HIV – Syphilis prevalence estimation using non invasive methods among MSM in Southem and Eastem Europe”
Destinatari: aperto al pubblico
Data: 05/02/2010
Luogo svolgimento: Verona University Hospital – Clinical Immunology Unit
Responsabile
Segreteria organizzativa
- CENTRO REGIONALE DI RIFERIENTO PER LA PROMOZIONE DELLA SALUTE - U.L.S.S. 20 REGIONE VENETO
- U.O. di IMMUNOLOGIA CLINICA - OSPEDALE POLICLINICO "Giovanbattista ROSSI" AZIENDA OSPEDALIERA UNIVERSITARIA INTEGRATA VERONA
- U.O. MICROBIOLOGIA VIROLOGIA E IMMUNOLOGIA - AZIENDA OSPEDALIERA UNIVERSITARIA INTEGRATA VERONA
Spese partecipazione
Edizioni successive
Note
STARTING: 8.30
- Presentation of the day schedule
Dr. Dunia Ramarli, Prof. Roberta Fontana
9.00
- Lab Training: Recovering of Oral fluid from the collection device, clearing of Oral fluid samples , storage
- 1st step of the Time Resolved Fluorescence Immuno Assay (TRFIA) for detection of Anti Treponema IgG (plate loading, 2h incubation time)
Dr. Paola Coato, Dr. Claudia Pizzoli, Dr. Monica Brentegani
9.30-11.30
- Rationale of TRFI and general application
- Presentation of pilot and survey results regarding syphilis
- Discussion on cut off values and discrimination of recent from previous infection.
Prof. Roberta Fontana, Drssa Elisabetta Tonolli, Dr.ssa Paola Coato
11.30-12
- Lab training: 2nd step of TRFIA: plate washing, 1st antibody addition, 2h incubation time
12.30-13.30
- Lunch time
13.30-14.30
- EIA Biorad on oral fluid sample (presentation of validation and survey results (discussion on cut off values and false negative results)
Dr.ssa Dunia Ramarli, Dr.ssa Paola Coato
14.30 -15.00
- Lab training: final step of TRFI (plate washing, 2nd antibody addition, 10min incubation, reading)
15.00-16.00
- General discussion on methods and results (all participants)
- Presentation of handbook - agreement on final version
- Possible application of tests in each country's context and local dissemination
16.00
- Ending
Allegati
- scarica il programma del corso [20 Kb]